ravo PNS-Blot 2 engl
ثبت نشده
چکیده
8, 16 or 24 Determinations Version 07/2015 Please pay attention to the differences in comparison to the previous version 10/2013 Complete revision: details on specimen added (page 5), information on performance data and interfering substances added (page 8). Modification and improvement of test procedure (page 6) Page 6: Procedure: Dilution of serum samples 1:200 in one step Sample incubation: only 30 minutes instead of 60 minutes Substrate incubation: reduced to 20 minutes
منابع مشابه
Panax notogingseng saponins suppress RAGE/MAPK signaling and NF-kappaB activation in apolipoprotein-E-deficient atherosclerosis-prone mice.
BACKGROUND Panax notoginseng saponins (PNS) extracted from the roots of panax notoginseng are free radical-scavenger, with an antioxidant property, capable of inhibiting expression of adhesion molecules and chemokines. This study was designed to test the effects of PNS administration in apolipoprotein (apo)-E-deficient mice on the activation of JNK, p38(MAPK), ERK1/2 and NF-κB, and the expressi...
متن کاملSFE-CO2 extract from Typhonium giganteum Engl. tubers, induces apoptosis in human hepatoma SMMC-7721 cells involvement of a ROS-mediated mitochondrial pathway.
Typhonium giganteum Engl. (BaiFuzi) is one of the herbs commonly used in traditional Chinese medicine against cancer. In our previous studies, 37 compounds were identified the SFE-CO(2) (supercritical fluid extraction with CO(2)) extract by GC-MS, including the four major components [β-sitosterol (40.22%), campesterol (18.45%), n-hexadecanoic acid (9.52%) and (Z,Z)-9,12-octadecadienoic acid (8....
متن کاملPanax notoginseng saponins improves the malignancy of chronic myeloid leukemia by inducing cell apoptosis and autophagy
In recent years, the anti-tumor effect of panax notoginseng saponins (PNS) has been widely acknowledged by multiple in vivo and in vitro experiments. However, few studies have focused on the effect of PNS on chronic myeloid leukemia cells. In this study, we mainly explored the effect of PNS on K562 cell apoptosis and autophagy, thereby exploring its potential application in the treatment of chr...
متن کاملPanax notoginseng saponins promote wound repair of anterior cruciate ligament through phosphorylation of PI3K, AKT and ERK.
Panax notoginseng saponins (PNS) are components derived from Chinese herb panax notoginseng and play important roles in the cure of wounds. However, how PNS plays this function is still unclear. In this study, we used MTT assay, wound healing assay, western blot, quantitative real time PCR and enzyme-linked immunosorbent assay to detect the effects of PNS on the proliferation, migration and exp...
متن کاملPeripheral nervous system insulin resistance in ob/ob mice
BACKGROUND A reduction in peripheral nervous system (PNS) insulin signaling is a proposed mechanism that may contribute to sensory neuron dysfunction and diabetic neuropathy. Neuronal insulin resistance is associated with several neurological disorders and recent evidence has indicated that dorsal root ganglion (DRG) neurons in primary culture display altered insulin signaling, yet in vivo resu...
متن کامل